FastQCFastQC Report
Thu 9 May 2024
EGAF00007335253

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335253
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1803455
Total Bases267.3 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN153470.8509777066796786No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACGGGCACTT89040.49371900047409006No Hit
GGGCCGGCTGACCCTGCGCAGATTAACTTTACGCAGGAACCCTTGGACTT82500.4574552733503192No Hit
AGGCGAGTCCGAAAGGAGTAGTCGATGGGAACCAGGTGAATATTCCTGGG67030.37167547845662907No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG38470.21331278019135494No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC37340.2070470291745566No Hit
TTACCTCTTCCGGTCTCAAGCCAAACAGTATCAAAGGCAATTCCAAGGTT33950.18824977612416166No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTTACAATGGAGAGTTTGATCCT29600.1641294071656903No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG21570.11960376055959257No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC18800.10424435319983032No Hit
CGGCCCCCCTTTACAATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTA18640.10335716721515092No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)