FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335257

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335257
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3576212
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN579551.6205694740692107No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC165450.4626403580100956No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC68400.19126382887815374No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT57870.16181926574822747No Hit
CGGCCCCCCAAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACG57260.16011355031524976No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA54750.15309495074676782No Hit
CGGCCCCCCGAACCGGGGGAACTGAAACATCTAAGTACCCCGAGGAAAAG51120.1429445457931465No Hit
AGGCTCTCACTGGATGACAGTGAGTGGTGGAGTCCGGAGGAGAAGCTGCG44870.1254679532421456No Hit
AGGCGGGCCTGCGGGCTGGGGACCTCATCACCCACATCAACGGGGAGTCA42310.11830954093325563No Hit
CCACTTCTACTTTACTCAAGACCTGCAGTATCAATGGCAGTTTCACAGTT40960.11453459694223944No Hit
GGAGGCAAGGTTAACACAGGCCCCCTGAGGAACATCCTTCAGGACAATGT36550.10220311323825321No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)