FastQCFastQC Report
Wed 8 May 2024
EGAF00007335261

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335261
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3716603
Total Bases555.1 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT600951.616933527740251No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG354180.9529669970131327No Hit
AGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGATGCTTT193230.5199102513774003No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG185500.4991116888190641No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT101340.27266834795107253No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC90880.24452436808558783No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT70000.18834403351662796No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC69650.1874023133490448No Hit
CGGCCCCCCAAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGC62780.1689176917739129No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG56140.15105191488033562No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT55750.15000256955074298No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG54870.14763481598653394No Hit
CGGCCCCCCAAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACG49890.134235483316351No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC48540.1306031340985303No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC47170.12691697229970486No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA45160.12150880790872741No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG44990.12105140097018702No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC39790.10706012990895182No Hit
CGGCCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGC37800.10170577809897909No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG37300.10036046357386032No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)