FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335263

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335263
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593031
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC311040.8656758040773932No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC183410.5104603884575446No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG172760.4808196756443237No Hit
NNNNNNNN112840.3140523975440234No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC99530.27700846444130317No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA69550.19356916208070568No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT66660.1855258137210617No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC54210.15087540296757807No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT51910.1444741222661313No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG51470.1432495294362893No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC47760.13292398534830344No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTAAACCTAGCCCCAAACCCAC44730.12449099381552789No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG44390.12354471753792272No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT40960.11399845979619992No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC38240.10642824957535853No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT36500.10158554156643793No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)