FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335275

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335275
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4004531
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC226590.5658340514781881No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC99730.24904289665880971No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC91330.22806665749372398No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG87980.21970113354098145No Hit
NNNNNNNN68270.17048188664290526No Hit
TCTATTAGCATTTTTGTGAAGAGGCCTCTGAGTCTGAGTCCTTTGTTAGC55840.1394420470212367No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT49300.12311054652841993No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA44490.11109915243507917No Hit
TGGCAATACCTTTTGGCTTCCAGGGAAGTTCAAGGCAATAGAGAAGGAGG43150.10775294285398215No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)