FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579284
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG279120.6095276030051859No Hit
AGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT183110.39986600525322297No Hit
AGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGAGTACG162240.3542911948680187No Hit
NNNNNNNN157110.34308857017822No Hit
TGGCACTCAGCACCCCCCTGCCTCTCCTCCTATTCTGCCTTCTCAGGAAC137860.301051430747689No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC132620.289608593832573No Hit
GGGCTGACTCCTGCAGCTGCTGCTCTAGCTCCTTCACACGGACCTTGAGC127100.27755430761664923No Hit
AGGCCGAAATGGAGCGGGCACTGCTGCAGGGAGAGAGGGAGGCAGAGCGG120310.2627266620720619No Hit
CGGCAGATAGGCCTTGACCACAAACATGGGGGTGCCGGCCACCTGGGACT111540.24357519647176282No Hit
AGGCTGTTCTGGGCATCAGCTCAGGCATTGGTTTAAGAGCTGGGGGATTA109820.2398191507668011No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC101380.22138832184245397No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC94770.2069537508483859No Hit
TGGCGGTGGACCTAAACCGTTTGATATGCCAATGGCGGATTTTCGCCGTG85730.18721267342230793No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT79350.17328036435390337No Hit
CGGCCCCCCGATGGATGGATAGATAGATAAGTGGATGGGTGGATAGATGG79310.17319301445378799No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG76020.1660084851692972No Hit
TGGCCCTGAGCCTGATCAGTGTGAGACAGAACTCAGCATTACTGTCCACC75810.16554989819369142No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGACG73710.1609640284376335No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC67620.14766500614506547No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT67300.14696620694414236No Hit
AGGCCCACAATGTTCCCACAAGGCACATCCTCGATGGGCTCCACGTAGCG63510.13868980390820923No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC61680.13469354597793018No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC61530.13436598385249746No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA59650.13026053854707417No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG55260.1206738870094102No Hit
CGGCCCCCCTGAGGCTGGGGAGCTTCCCAGCATTGGGGAGGCCACCGCAG54280.11853381445658318No Hit
CGGCCCCCCAGTGGGCCAATTTCATCCTTATCTTAGTTAACCTTGGTGCA52240.11407896955069832No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGACGG51870.11327098297463097No Hit
AGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCAGCCACT50840.11102172304665969No Hit
ATGTCATGGCAGGTGAGGGAGAGGGAGAAAAGGAGGCTGACTCGAGTTAC50530.11034476132076544No Hit
CGGCGAGGCACTGTAGTATCAGTCAGTCCAGGAATATCCTTCTCTCCTTT49370.10781161421741915No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA48420.10573705408967865No Hit
CGGCCCCCCACAGTGAGACCCTGTGTCACATACACGAGAAGAAAGCAAGC47360.10342228173662084No Hit
CGGCCCCCCACGGGGGCCCGCACAAGCGGTGGAGCATGTGGTTTAATTCG47300.10329125688644775No Hit
AGGCGGCTGATGTCGGCATCTCGCTGTCCCAGGCAGAAGCCTCAGTGGTC45950.10034319775755338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)