FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4579284
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG262190.5725567577813475No Hit
AGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT178520.38984260421498207No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC139400.3044144019021314No Hit
GGGCTGACTCCTGCAGCTGCTGCTCTAGCTCCTTCACACGGACCTTGAGC125530.2741258240371202No Hit
AGGCTTTAGTGGCGCAGCTAACGCGATAAGTAGACCGCCTGGGGAGTACG117230.25600071976317695No Hit
AGGCTGTTCTGGGCATCAGCTCAGGCATTGGTTTAAGAGCTGGGGGATTA115150.2514585249571767No Hit
CGGCAGATAGGCCTTGACCACAAACATGGGGGTGCCGGCCACCTGGGACT99570.2174357388622326No Hit
TGGCACTCAGCACCCCCCTGCCTCTCCTCCTATTCTGCCTTCTCAGGAAC97660.21326478113172279No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC92380.2017345943164914No Hit
AGGCCGAAATGGAGCGGGCACTGCTGCAGGGAGAGAGGGAGGCAGAGCGG88880.19409147805639484No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC81440.17784439663493246No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT72740.15884579335983529No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC67860.14818910554575782No Hit
TGGCCCTGAGCCTGATCAGTGTGAGACAGAACTCAGCATTACTGTCCACC67390.14716274421940198No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC64250.14030577706034392No Hit
CGGCCCCCCGATGGATGGATAGATAGATAAGTGGATGGGTGGATAGATGG62480.13644054398023797No Hit
TGGCGGTGGACCTAAACCGTTTGATATGCCAATGGCGGATTTTCGCCGTG61480.13425679647735322No Hit
CGGCCCCCCACAGTGAGACCCTGTGTCACATACACGAGAAGAAAGCAAGC61220.13368902212660322No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC58680.128142303469276No Hit
AGGCCCACAATGTTCCCACAAGGCACATCCTCGATGGGCTCCACGTAGCG56830.12410237058893922No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGACG56130.12257374733691993No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT54750.11956017578293898No Hit
CGGCCCCCCAGTGGGCCAATTTCATCCTTATCTTAGTTAACCTTGGTGCA53150.11606617977832344No Hit
CGGCCCCCCTGAGGCTGGGGAGCTTCCCAGCATTGGGGAGGCCACCGCAG51870.11327098297463097No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG50300.10984249939510193No Hit
CGGCGAGGCACTGTAGTATCAGTCAGTCCAGGAATATCCTTCTCTCCTTT49300.10765875189221721No Hit
GCTCTATCTCCTCCTCAAGCTACCCCTGCATCTCCCTCCTTCCCTCAGTG48990.10698179016632293No Hit
AGGCGTTCAGCTGCTTCAAGATGAAGCTGAACATCTCCTTCCCAGCCACT46710.10200284585974576No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA46170.10082362220818801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)