FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283236
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC411751.2540980910297035No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC284850.8675891711713688No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG165710.504715469737783No Hit
NNNNNNNN152410.4642066546541278No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC131000.3989965996961534No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT114130.34761436582688543No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG99930.30436435273005047No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC72210.21993545392411631No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA70290.214087564829333No Hit
CGGCCCCCCAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA50630.154207617119208No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT44700.13614616798792412No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT37350.11375971754695673No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC34330.10456147532495379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)