FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3283236
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC415811.2664639398447142No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC271930.8282377508043894No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG137370.4183981900783252No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC131940.4018596287321411No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT106380.32400960515783817No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG104270.3175830187047169No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC76580.2332454931658888No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA57350.1746752289509496No Hit
CGGCCCCCCAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTA57120.1739747005698037No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT44530.13562838614099018No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT36990.11266323834168485No Hit
AGGCCTTGACCTTTTCAGCAAGTGGGAAGGTGTAATCCGTCTCCACAGAC33690.10261217896002603No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)