FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335291

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335291
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9494745
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT511330.5385400029174032No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG360460.3796415806848947No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC359930.37908337717337326No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA331090.3487086804332291No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG309490.32595925430330147No Hit
CGGCCCCCCAATGAAGTGCACATGGTGGATGCCTTGGCAGTCAGAGGCGA292590.3081599347849784No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG265420.2795441057132129No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA253870.26737948201873774No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG252420.2658523214683491No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG222110.23392939989436262No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT212830.22415557237187517No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAATGAAGTGCACATGGTGGATGC198840.2094211060960563No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG190630.20077421773833842No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT184870.19470770410369104No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT184170.19397045418281375No Hit
CGGCCCCCCATTGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAACG162130.1707576138169061No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT151260.15930917575985454No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGAACGCTGGCGGCAGGCTTA150080.15806638303608997No Hit
NNNNNNNN144210.1518840158424476No Hit
TGGCAATCAGGTGTTGAGTGTAAGTGCACAAGGGAGCTTGACTGTGAGAC140930.14842947335605117No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC101120.10650101714158727No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)