FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335301

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335301
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4432734
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2979596.7217884041767455No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC291850.6583972780681178No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG267220.6028333755194875No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG252210.5689716549650847No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC209290.4721465352985313No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG171420.3867139332069102No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG141780.31984775084631745No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC139780.31533586269782937No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG136820.3086582682380671No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC122810.27705249175790836No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT121830.2748416665651492No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT117520.26511854760515746No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA112500.25379370835245246No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG108440.24463457541102174No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG99540.22455667315024994No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA97550.22006734444250434No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC86880.1959964211703206No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA82740.18665681270295037No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG79090.17842261683195967No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGACCTCAGATCAGACGTGGCG77660.17519661680579074No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT76280.172083413983334No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT69220.1561564488191712No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAATTGAAGAGTTTGATCATGGC65560.1478996935074381No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC64670.1458919032813609No Hit
AACTAGGCAAAATGGTGCCGTAACTTCGGGAGAAGGCACGCTGATATGTA62770.14160560954029727No Hit
NNNNNNNN59110.13334885422856413No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC58850.13276230876926068No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG57830.13046124581353177No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGACACGGTCCAGACTCCTACGG55870.12603959542801352No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGACCAAGGAGTCTAACA54390.12270079819813236No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC54250.12238496602773817No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG49850.11245881210106448No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG47940.10814995891925841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)