FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5446951
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT2239904.112208830224469No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT205330.3769631854591679No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC171650.315130428013764No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG150960.27714587482061065No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC146810.2695269335083058No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG138810.2548398177255496No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT118610.21775485037409No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG112150.20589500437951433No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG107790.19789052627791218No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG105220.19317229033270172No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG98510.18085347196991491No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG93210.1711232577638389No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC87480.1606036110844397No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA69030.12673145031045807No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGATGTTCGCCGACCGTTGACTA67630.12416120504847575No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC62220.11422904300038683No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT61860.11356812279016279No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT56390.10352580737370319No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)