FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335306

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335306
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5227434
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT684041.3085578890139982No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC380350.7276036388025177No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG215950.41310899381991245No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGATGTTCGCCGACCGTTGACTA201730.3859063548195922No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA154280.29513524226226484No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC124750.23864481120182482No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC120270.23007464082760298No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT119860.229290317199605No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTAAACCTAGCCCCAAACCCAC80750.1544734950264317No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT66460.12713694711401427No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC58970.1128086935196121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)