FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1593276
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT799285.016582186639352No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC58350.3662265671484413No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG53230.33409151961116595No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG53160.33365217325811725No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG52150.3273130330212719No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT44220.2775413675973278No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG35900.22532191534925522No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG34270.21509142169969295No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT26440.16594739392296123No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG26280.16494317368742137No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT22550.1415322894463985No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA21720.1363228969745355No Hit
NNNNNNNN20560.12904230026687152No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAATTGAAGAGTTTGATCATGGC19710.12370738026556605No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC18720.1174937675581632No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG18460.1158619096754109No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGATTCCTGAAGCTGACAGCATTC18030.11316306779239754No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG17150.10763985649692835No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG17150.10763985649692835No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC17030.10688669132027345No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC16920.1061962899083398No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGCACAATGGGCGCAAGCCTGA16380.10280704661339278No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)