FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4174850
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGAGACCTT438131.0494508784746757No Hit
GTGGTCCCGGCAGATTCACAGCAGATTCCACGAGCCCGCTGCTACTCGGG160000.3832473022982862No Hit
GGAATTCCTGGTGTAGCGGTGGAATGCGCAGATATCAGGAGGAACACCGA156770.3755104973831395No Hit
AAGGCTGCGTTAAGCCTCGGGGAGTTGCCAATAAAGCGTTGATCCGAGGA154800.37079176497359184No Hit
TCTCCCCTACAGCACTCAAGTTATGCCCGTATCGCCTGCACGCCCGAAGT131990.31615507143969246No Hit
TTTGTATAATGGGTCAACGACTTGGTCTGTCTAGCAAGCTTAAGCCGTTA104500.25030839431356816No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA69770.16711977675844641No Hit
GGTAAGCCTAAATACTCAAGTTGACCGATAGCGGATCAAGTACCGTGAGG68140.16321544486628262No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA67830.1624729032180797No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG66980.16043690192462004No Hit
GCGCAGAACCACTCGACCAGTGAGCTATTACGCACTCTTTCAAGGATGGC63690.15255637927111154No Hit
AGGCCACACGAGTGAGAATGCAGGCATGAGTAGCGAAAGACGTGTGAGAA57900.1386876175191923No Hit
AGGCTGAGGGAACCTTTGGGCGCCTCCGTTACATTTTAGGAGGCAACCGC57560.13787321700180843No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG52460.1256572092410506No Hit
GTACGCCGTCACCCCTCAAGGGGGCTCCGACTGCTTGTAGGCGTCCGGTT51650.12371701977316549No Hit
CTCCCCTACAGCACTCAAGTTATGCCCGTATCGCCTGCACGCCCGAAGTT47860.11463884929997487No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG47190.11303400122160077No Hit
GGCGCAGAACCACTCGACCAGTGAGCTATTACGCACTCTTTCAAGGATGG46950.11245913026815335No Hit
NNNNNNNN46860.11224355366061056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)