FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335323

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335323
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3529404
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGATGCTTT89990.25497222760556737No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCAGCATAAG57440.16274702471012104No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC53200.1507336649473962No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCAGCATAA52370.14838199310705152No Hit
ATACCTCTCTCACACTCAAGACACCCAGTATCAAAGGCAGTTCCAGAGTT51400.14563365372737153No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT46640.13214695738997292No Hit
TGGCCGGTCCGCCTCACCGCGAGTACTGGTCCGGCTGGGCCTTTCCTTCT45830.1298519523409618No Hit
GGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG40450.11460858547222139No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG39200.11106691101387091No Hit
NNNNNNNN36520.1034735609751675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)