FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3531777
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT504121.427383438988362No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT412231.1672027990442206No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG309270.875678164278209No Hit
NNNNNNNN244580.6925125793616075No Hit
CGGCCCCCCTATACGGAGAGTTTGATTCTGGCTCAGAACGAACGCTGGCG115440.3268609541315887No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC100740.2852388471865579No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA77140.21841696120678064No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA74850.21193297311806492No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG70170.1986818533559735No Hit
CGGCCCCCCTGTGATCAAGCTACTAAGTGCAATACGGTGAATGCCTTGGT65830.18639342178172633No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATACGGAGAGTTTGATTCTGGC54290.15371865211195385No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC53510.15151013215160528No Hit
CGGCCCCCCGGTCAAGCTACTAAGGGCAAGCGGTGGATGCCTTGGGACTT45340.1283773012848773No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG42860.12135534038530744No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG37460.10606558681366349No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)