FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3828694
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA1258713.287570121822219No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT729911.9064203093796477No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG376580.9835729885961113No Hit
NNNNNNNN364520.9520739970339755No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT293180.7657441414748737No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT251730.6574826820842825No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA246500.6438226716473032No Hit
TGGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTT246480.643770434513701No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT228750.5974622155753372No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG202950.5300763132284795No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT194420.5077971757471347No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG189160.49405880960975207No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT184140.480947289075596No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT178270.4656156903633459No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA144570.37759612024361305No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC137560.35928700491603666No Hit
CGGCCCCCCAACGCGCAGAACCTTACCAGCGTTTGACATGTCCGGACGAT86010.2246457930563268No Hit
CGGCCCCCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAA72800.1901431663120636No Hit
CGGCCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGT57390.14989445487155673No Hit
GGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTTG53740.14036117798915243No Hit
CGGCCCCCCTGGGCGGCGGCTTCACGGTTTTTCGGGTCCTCTAGGCTCGG53560.1398910437867325No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGGCGGCGGCTTCACGGTTTTT53370.13939479101751145No Hit
CGGCCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGC40890.10679881964972912No Hit
CGGCCCCCCACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGGGAATAT40130.10481380857284495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)