FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335353

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335353
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330800
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA834081.925925925925926No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT514051.1869631476863396No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT337890.780202272097534No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT275320.6357255010621594No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT208560.4815738431698531No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT203010.46875865890828483No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG163870.37838274683661216No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA163810.3782442043040547No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG146910.33922139096702686No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG142070.3280456266740556No Hit
TGGCGGACATCCGGGCGGTTGCGCCGGACGGCGTTTTCATGTCGAACGGT137530.3175625750438718No Hit
GGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG133190.30754133185554633No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT128910.2976586311997783No Hit
ACACCTCTCTCATACTCAAGACACCCAGTATCAAAGGCAATTCCGAGGTT117410.2711046457929251No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT109170.2520781379883624No Hit
CGGCCCCCCGAACAGCCATTCTCATCCAAACTCCCTGAAGCTTCACCGGC105670.24399649025584189No Hit
AGGCCCTCTTGAAGCAGGGGCAAGATAACCTCTCAAGTGTGAAGGAGACT98710.22792555647917243No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG95400.22028262676641727No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG90510.20899141036298144No Hit
CGGCCCCCCTGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTA84620.195391151750254No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA81010.18705550937471138No Hit
CGGCCCCCCGGTCACCTGATGAGCGTGAGAGGGCAGGAGTAAAGCATATG79930.18456174378867646No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG77730.17948185092823496No Hit
CGGCCCCCCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG72280.16689757088759583No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC70640.16311074166435763No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT65330.1508497275330193No Hit
NNNNNNNN64590.14914103629814354No Hit
AGGCTGAACAGCCAAACAAAATCTAAGAGGCGAAGTAAATACCCAGAGCC62710.14480003694467533No Hit
CGGCCCCCCTTGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC62510.14433822850281702No Hit
CGGCCCCCCTGTGGTTCTGCTGCCGCAGGATTGGCCAGTGAGAAATGTTG62040.14325297866445No Hit
CGGCCCCCCATACCCTGGTAGTCCACGCCGTAAACGATGATAACTAGCTG62020.14320679782026416No Hit
CGGCCCCCCAGAAACTGGGGCAGTTCTGGCTGACTTGAACTTGTTACCTG61930.1429989840214279No Hit
CGGCCCCCCTATGGCAAATAGGAAGTGAACACTTACTATGGGCAGGCCCT61590.14221390967026878No Hit
CGGCCCCCCAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTC61290.1415211970074813No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG60990.14082848434469383No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC60300.13923524522028263No Hit
CGGCCCCCCGATCCGTTCCTTCGGTGCATCCACCGCGAACCCGTGGTTCT59580.13757273482959267No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG57850.13357809180751826No Hit
TGGCTGCTATAAAAAATGTCTTATCACCCAATTCAGATACAATAATAACT57400.13253902281333704No Hit
AGTTGCCGAACCAGGCCGCCTCACAGCCAGGAAATTTGGAAATCCTAGCC57060.1317539484621779No Hit
CGGCCCCCCAACGGAGAGTTTGATTCTGGCTCAGGACGAACGCTGGCGGC56980.13156922508543456No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT55550.1282672947261476No Hit
CCCAGCATCACTAACTCAAGCGTCCTGCTCTCTGACCAATCTACTCTACT52610.12147871063083032No Hit
CGGCCCCCCGACCAGCCACACTGGAACTGAGACACGGTCCATACTCCTAC50560.11674517410178258No Hit
CGGCGCTGAAGGCCTGACGCCGGCGGTCCGGGCGATCGCTCGGGTTCAGC50250.11602937101690218No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC50100.11568301468550844No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA48150.11118038237738986No Hit
CGGCCCCCCTCTGAGACACGGCCCAGACTCCTACGGGAGGCAGCAGTGGG47820.11041839844832364No Hit
CGGCCCCCCGGAATATTGGACAATGGGCGAAAGCCTGATCCAGCAATGCC47440.10954096240879284No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC46640.10769372864135955No Hit
CGGCCCCCCACGCTAATGAACCGGCGGCCGGAAGGCTGTTTGCCCAAGGC44920.10372217604137804No Hit
CGGCCCCCCGGCCTTCTGGCTGAGATCCATTTTGAAAAACAGCCTTACTC43980.10155167636464395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)