FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4330800
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA561171.2957652165881592No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT358630.8280918075182414No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT317750.7336981620024015No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT272630.629514177519165No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT178980.41327237461900806No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT165490.3821233952156645No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG141600.32696037683568857No Hit
ACACCTCTCTCATACTCAAGACACCCAGTATCAAAGGCAATTCCGAGGTT123320.2847510852498384No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT118600.2738524060219821No Hit
TGGCGGACATCCGGGCGGTTGCGCCGGACGGCGTTTTCATGTCGAACGGT116400.2687725131615406No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG109850.25364828669068074No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT108310.2500923616883717No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG101650.2347141405744897No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA99690.23018841784427818No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG93940.21691142514085157No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG85850.19823127366768262No Hit
CGGCCCCCCGAACAGCCATTCTCATCCAAACTCCCTGAAGCTTCACCGGC84590.19532188048397525No Hit
GGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG83840.19359009882700656No Hit
CGGCCCCCCTGACCAGCCACACTGGAACTGAGACACGGTCCAGACTCCTA72060.16638958160155168No Hit
CGGCCCCCCGGTCACCTGATGAGCGTGAGAGGGCAGGAGTAAAGCATATG71300.16463470952249007No Hit
CGGCCCCCCGATCCGTTCCTTCGGTGCATCCACCGCGAACCCGTGGTTCT65920.15221206243650134No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC60110.13879652720051722No Hit
AGGCCCTCTTGAAGCAGGGGCAAGATAACCTCTCAAGTGTGAAGGAGACT59360.13706474554354853No Hit
CGGCCCCCCTTGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC58810.13579477232843815No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA55600.12838274683661216No Hit
AGGCTGAACAGCCAAACAAAATCTAAGAGGCGAAGTAAATACCCAGAGCC54950.12688186940057264No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT54770.12646624180290014No Hit
CGGCCCCCCTGTGGTTCTGCTGCCGCAGGATTGGCCAGTGAGAAATGTTG53000.12237923709245405No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC52720.12173270527385241No Hit
CGGCCCCCCGCAGCAGTGGGGAATATTGCACAATGGGCGCAAGCCTGATG51900.11983929066223331No Hit
CCCAGCATCACTAACTCAAGCGTCCTGCTCTCTGACCAATCTACTCTACT50730.11713771127736215No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG49970.11538283919830054No Hit
TGGCTGCTATAAAAAATGTCTTATCACCCAATTCAGATACAATAATAACT49350.11395123302853977No Hit
CGGCCCCCCATACCCTGGTAGTCCACGCCGTAAACGATGATAACTAGCTG49150.11348942458668146No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG46560.10750900526461622No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC46090.1064237554262492No Hit
AGTTGCCGAACCAGGCCGCCTCACAGCCAGGAAATTTGGAAATCCTAGCC45480.10501523967858134No Hit
CGGCCCCCCAGATAGCTGGTTCTCCCCGAAAGCTATTTAGGTAGCGCCTC44300.10229056987161725No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT44140.1019211231181306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)