FastQCFastQC Report
Thu 9 May 2024
EGAF00007335363

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335363
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4857797
Total Bases725.7 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC1351282.781672432997921No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC508211.046173810885881No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA275530.567191259741813No Hit
CGGCCCCCCAACACGGACCAAGGAGTCTAACATGTGTGCGAGTCGGTGGG274600.5652768116905668No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG266460.5485202448764327No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT243870.5020176841477731No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG210640.4336121908758229No Hit
GGGCTACGGACGTAGAATGTCCTCTCGCGGGGGCTTTTCCCTAGGCGGCG194420.40022257002505457No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT179420.3693443756501147No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG165010.3396807235872557No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAA158260.3257855361185327No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT152780.31450470244022133No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT147400.3034297233910762No Hit
AGGCCATGGAAGTTTGAGGCAATAACAGGTCTGTGATGCCCTTAGATGTT137860.2837911917686145No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT91570.18850108392754988No Hit
CGGCCCCCCTGCGACCCCAGCTCAGGCGAGAACACCCGCTGAGTTTAAGC89340.18391052569714214No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT87800.18074036440798164No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT86850.17878474543090211No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC86560.17818776700631994No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC86160.17736434848965488No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC79240.16311920815134925No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT63400.13051183489141271No Hit
CGGCCCCCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCGCGGTAATTCCA58910.12126896204184737No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGACCAAGGAGTCTAACA58790.12102193648684785No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA57810.11900456112101843No Hit
CGGCCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACT56200.11569030159144156No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG55260.11375526807727865No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCATGGCACATGCAGCGCAAGTAG50330.10360663485938174No Hit
CGGCCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATT49430.10175394319688533No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)