FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335365

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335365
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5008631
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT573291.1446041842571353No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC299140.5972490287266121No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT274930.5489124672989486No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC136880.27328824982315525No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG113380.2263692414154686No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG89970.17962992282721565No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT86450.17260205433380899No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG71850.14345237251456536No Hit
NNNNNNNN69700.13915978238364934No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG61110.12200938739547793No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC59060.11791645261948824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)