FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5393489
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT454050.8418483842277235No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT432130.8012067884072814No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC404800.7505345797497687No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA246590.45719941210596704No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG242710.4500055529917647No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC239090.4432937566017099No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG218050.40428375769376745No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT188280.3490875757788697No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTAAGTGGTAGGGGAGCATTGT150940.2798559522416751No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG143570.2661913280994918No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG103910.19265822179298037No Hit
NNNNNNNN88360.16382716271415404No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT78620.1457683514326255No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG76010.14092918331714407No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC70430.1305833756219768No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG66190.12272204504357014No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC64050.11875429800635544No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT60720.1125801869624653No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG59040.10946532012951173No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT58300.10809329545309167No Hit
CGGCCCATGGGTCCTCCTCGCCCTCGGCCTCCACGACCATTGCCACCACC54300.10067694585082124No Hit
AGAAGGAGGTGGTGGGCCAAGAAATCCACCTGCCTGCTTGGTGGCGGCTC53990.10010217875664527No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)