FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335368

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335368
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5393489
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT430920.7989633426525946No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC366570.6796528184260688No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT317350.5883946365701311No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA198750.36849987086281255No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG170070.3153246442145335No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT159280.295319041162409No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC156420.2900163511967856No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG149530.27724168900687474No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG110890.20559975184894233No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTAAGTGGTAGGGGAGCATTGT110710.20526601611684014No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90570.16792469586940847No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT70730.13113960184214707No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC66760.12377887486189366No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG65510.12146126561118416No Hit
CAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAAT58550.10855681730323359No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG57430.10648023941459786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)