FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4835767
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGAGGACTT421620.8718782356552746No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG355340.734816214263425No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG309350.6397123765475052No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC163810.33874667658718877No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT163740.3386019218874689No Hit
AGAACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGCGCCCTT159350.3295237342907547No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT125950.2604550632815849No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT120010.2481715930482176No Hit
AGGCTGAGGGAACCTTTGGGCGCCTCCGTTACATTTTAGGAGGCAACCGC111090.22972570845534948No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA100570.2079711450117427No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG92840.19198609031411148No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC92390.19105552438734125No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG89840.18578231746897647No Hit
CGGCAGACATGACAAGGAGGGGCCCACAACACCGCACATGCAACCCCTGC89760.18561688352643954No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC84630.17500843196125868No Hit
AGAACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGCCCTTTC83370.17240284736630196No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAACTGAAGAGTTTGATCATGG81320.16816360258879304No Hit
CGGCCCCCCTAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGC74190.15341930246018884No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT72470.14986247269564476No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG72390.14969703875310783No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC60760.1256470793568011No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTTCAACTTGAGAGTTTGATCCT60710.12554368314271552No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC56190.11619666538937876No Hit
TGGCGCAGTGAGGTGCGGGCATATAGACGAACCAGTGTGGATGCTGGACC54780.11328089215216532No Hit
NNNNNNNN54690.11309477896681126No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG52010.10755274189182398No Hit
TGGCAATCAGGTGTCGAGTGCAAGTGCACAAGGGAGCTTGACTGTGAGAG48570.10043908236273584No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)