FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4327038
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT547531.265369058464474No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT504341.1655548206417417No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG143120.3307574373046874No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG122940.2841204537607481No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA112220.25934600065911134No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTAAGTGGTAGGGGAGCATTGT82380.19038427672694347No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG76990.1779277186842362No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG66770.1543087904474146No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT66140.15285282911774753No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAACTGAAGAGTTTGATCATGG62700.14490281804781932No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC56490.1305511992268152No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC49020.11328765774647692No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGTCAAGTAATGAAGTGCACATGG44550.1029572654550295No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)