FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659416
Sequences flagged as poor quality0
Sequence length8-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT4044338.679907524891531No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT556161.1936259823119464No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG338910.7273658329713423No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC332530.7136731298514664No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG309320.6638600202257107No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC285420.6125660383189653No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACACGGTCCAGACTCCTACGGG255910.54923192091026No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGGTCCAGACTCCTACGGGAGG226530.48617680842406No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG222260.4770125698156164No Hit
GTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGT209100.44876868689123267No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG198430.42586882132868153No Hit
CGGCCCCCCTGAGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGA143830.3086867538764515No Hit
CGGCCCCCCGACACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATA143040.3069912624243038No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT141030.3026774170840294No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC120100.2577576245606746No Hit
CGGCCCCCCACGGTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTG113450.24348544967867217No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAATTGAAGAGTTTGATCATGGC98660.21174327426441425No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT81420.17474292915678702No Hit
AACTAGGCAAAATGGTGCCGTAACTTCGGGAGAAGGCACGCTGATATGTA81350.17459269573697647No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACGGTCCAGACTCCTACGGGA77370.16605085272489084No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG73530.1578094765524263No Hit
TGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATAAGCGTCGGTA69640.14946079079438282No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT68290.14656343198375077No Hit
CGGCCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGT65340.1402321664345918No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG61820.13267757160983265No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC59930.12862126927494777No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT59550.12780571642454763No Hit
TCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGTG56390.12102375061595702No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGGCGCAAGCCTGATGCAGCC55540.11919948766111461No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG54930.11789031071705122No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCGGTTAAGCGACTAAGCGTACAC51290.11007817288690255No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA50730.10887630552841815No Hit
AGGCAAATCGTTTAATCCCATTGCCTGGCGGATAAGTTGCGGCTTAACGC50410.10818952418071277No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT49160.10550678454123864No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT46710.10024861484786934No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)