FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335395

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335395
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4040077
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNN971682.405102675023273No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC167160.41375449032283296No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT86740.2146988782639539No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC77140.19093695491447316No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC54380.1346013949734126No Hit
GGGCAGGCACATGGGCATAGGTAAGCGGAAGGGTACAGCCAATGCCCGAA51160.12663124984994098No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG48110.11908188878578305No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA46380.11479979218217871No Hit
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG43240.10702766308661939No Hit
TGGCTCTGGAGAAGTCTCTTGTCCGGCTCCTTCTGCTTGTCCTGATACTG42390.10492374279005078No Hit
GGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT41790.10343862258070824No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)