FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335405

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335405
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5770224
Sequences flagged as poor quality0
Sequence length8-150
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG3736306.4751385734765226No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT1199172.078203549810198No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT844931.46429324060903No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGCA477210.8270216199579081No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG436080.7557418914759635No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATA390390.6765595235124321No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT359140.6224021805739257No Hit
GCCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATC356260.6174110398487129No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG320650.5556976644234263No Hit
TCTCAAAGAGGTAAGAGTGAGGCTGGCCTGGCTGAGTTCAGCTCTATGGG248270.4302605930029753No Hit
GAGCTCACTCTCTCCCCAGGAATCTCCCCGTGGTGGGTGCACACACTCAC222530.38565227277138636No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG194050.3362954367109492No Hit
CCATCAAACCTTTTTCAAAGGCAAAGGTGACCTTCAGGACATGTCATGCT173140.3000576754039358No Hit
TGAGCTCACTCTCTCCCCAGGAATCTCCCCGTGGTGGGTGCACACACTCA171150.2966089358056117No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC170050.2947025973341763No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG152870.264929056480303No Hit
CCCTGATTTCATGGCTCAAGACCCCAACTATAAATTAGCCCTTCCCACCT137830.23886421047085868No Hit
CGGCAGACATGACAAGGAGGGGCCCACAACACCGCACATGCAACCCCTGC131570.22801541153341706No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGACGGCGGAGTCCTTGCGTCT131400.2277207955878316No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG129230.22396010969418173No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT126350.21896896896896897No Hit
NNNNNNNN122410.21214081117128208No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT117200.2031116989565743No Hit
CGGCCCCCCACGCGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGA111510.19325072995433107No Hit
GGATGGGGCTGTCTTCACGCCGCTCAGCGAGAGCATCACGGATTGCTTGT109570.1898886421047086No Hit
AAGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGC107100.18560804571884904No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG105150.18222862751948626No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACGCGCAGAACCTTACCAGCCCT98510.1707212752919124No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGCGTAATGACTTCCCCGCTGTC95500.16550484002007548No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACAACGGAAGGCGTTAGGGAAGA94960.1645690011340981No Hit
CCGAATTCCTTAACCACAGTTCACCCGAACGCCTTAGTATTTTCAACCTG91850.15917926236485794No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC91650.15883265537005148No Hit
TGGCTCAGTTGACCTGGCTGATGATGGTGTCCGCGAATTCGCGCATGCTC89710.15547056752042904No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC89360.15486400527951774No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA88010.15252440806457426No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCTTACGCAGCCTGCACTCTTA84440.1463374732072793No Hit
CACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTTG83640.14495104522805352No Hit
TATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAGGAGACA79030.13696175399776508No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGCGAAGGGCGACCCGTGAGGGC77700.13465681748230224No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA77290.13394627314294905No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC75600.13101744403683463No Hit
CGGCCCCCCACAACGGAAGGCGTTAGGGAAGACCTTGACAGCATTCTCGA74350.12885115031929437No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAATTGAAGAGTTTGATCATGGC73910.12808861493072018No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGGAAAGATTTATCGCCGAAAGA70760.12262955476251874No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA69040.11964873460718335No Hit
CTTTACAACCCTAGGGCCTTCATCACTCACGCGGCATGGCTGGATCAGGC68930.11945810076003983No Hit
AGGCCTATGACGCGGCGGGGGCCGAGGTGTTGAGCCGGCTGGATATCTTC68390.11852226187406244No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG65970.1143283172369045No Hit
CGTCTTCCACGGTTCTCAAGGGAATGCTCGTTTTGAGGTGGGTTTCCCGC65860.11413768338976095No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAGTTTCCAAGGGCGCATGGTGG65670.11380840674469483No Hit
TCTAATCTTTGTCCCTAGAATAAAGATCGGTGGCAGGTAGTAGTGACAGC63910.11075826519039815No Hit
GTTAGCTGCGCCACTCAAGCTCTATGAGCCCGAACAGCTAGTTCTCATCG63700.11039432784585139No Hit
GGAAATCTGGCCACTGGGCCAAGAAATGCCCTCAGCCCGGGATTCCTCCT63200.10952781035883528No Hit
CAACAAAGCCTGTCCAGACCGATGTGCAAACACGGCCTGCGGATGCAAGC61450.10649499915427894No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGCTGGTCTGAGAGGATGACCA59860.10373947354556773No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)