FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2013112
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT351541.7462515746764213No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG142760.7091508073072934No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT88680.44051200330632373No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT71870.3570094460715549No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA62730.31160710382730816No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34550.17162482763005735No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT29960.14882430783781528No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG23470.11658566438429656No Hit
GCCAGGATCAAACTCTCAAGTTGGACTTGAAACCTTTGAACCGGCTGATC22170.11012800082658093No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG21030.10446512662981493No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG20860.10362066293380598No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT20700.10282587357285636No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG20640.10252782756250026No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)