FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335409

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335409
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4475838
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT1969984.40136573307613No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA193550.4324329879678398No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT181730.4060245254631647No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG176120.39349055975663105No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT169100.37780634598481894No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC124920.27909857327275917No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA123280.2754344549556977No Hit
CCGGAAGCCACGCCTCAAGGGCACAACCTCCAAGTCGACATCGTTTACGG100410.22433787818057757No Hit
NNNNNNNN98670.22045033801491473No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG98630.22036096927547422No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT94190.21044103919757598No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG93170.20816213634184255No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG86550.1933716099644357No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAATTGAAGAGTTTGATCATGGC80520.17989927249377657No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACTGGAACTGAGACACGGTCC75110.16781215048444562No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC72920.16291921200007686No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA67870.1516364086457106No Hit
AGGCGTGGCTTCCGGAGCTAACGCGTTAAGTCGACCGCCTGGGGAGTACG66520.14862021368959286No Hit
GGAACTCCGAGTGTAGAGGTGAAATTCGTAGATATTCGGAAGAACACCAG65720.1468328389007824No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT63490.14185053167697312No Hit
TATCAGATGCAGTTCCCAGGTTGAGCCCGGGGATTTCACATCTGACTTAA61750.13796299151131028No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG61700.13785128058700963No Hit
TTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGT59850.13371797638788535No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGCAGTGGGGAATATTGCACAAT55550.12411083689802893No Hit
CATGTAGCCTAGAGCTGCCTCCTTAAATATTTTAAGTTTGGCCTAAAGGG55340.12364165101596616No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT55030.1229490432853021No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTGCACAATGGGCGCAAGCCTG55020.12292670110044196No Hit
TCTCCCACTGCATGGCCCTACATGGGAAGGTGGAAAAGCTGCTTGTAGAT54240.12118401068135173No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC52680.11769862984317125No Hit
GGGGATTTCACATCTGACTTAACAAACCGCCTGCGTGCGCTTTACGCCCA52250.11673791589418563No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCAGCCACACTGGAACTGA50420.1126492960647816No Hit
GTACTCCCCAGGCGGGGCACTTAATGCGTTAGCTACGGCGCGGAAAACGT49380.11032570883932798No Hit
GCTCCACTCAGGGACTCAAGGTACAGATGGAGGATGGGGATGCAGCAGAG49370.11030336665446784No Hit
AAACTTTTTCTGTAAAGGGACAGAGAACTCTTTTAGACTCTGCAGGCCAT47350.10579024531272134No Hit
CGGCTCACTGCAACTTCTGCCTCCCAGGTTCAAGTGATTATCCTGCCTCA47320.10572321875814093No Hit
CTTGCACTCTCGGGCTCAAGTGATCTGCCCACCTCGGCCTCCCGAGGTGC47020.105052953212337No Hit
CGGCAAAATGCGTCCTTTTGGATGGCTGGGACCCAGCCTAGGGCGTAGCT46830.1046284516999945No Hit
ATTTCGGAGAGAACCAGCTATCTCCAAGTTCGATTGGAATTTCTCCGCTA45920.10259531287772257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)