FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4233519
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT746881.7642060895439466No Hit
NNNNNNNN323710.7646357557389019No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA295860.6988512393590297No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG159080.3757630472427312No Hit
GTGTAATTAAGGATCTCAAGATGAGATCATCCTGAATTATCTGAGAGAGG109820.25940594573923015No Hit
CTCCGCAGCTTGCCTACTACATGATCGGTTCACACACTCACCCACCCCAG94160.22241544209439001No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA88250.208455424435322No Hit
CGGCAAGCCGCTCGGTGAAATCGCGCTCAATGCGGACAAGACCTACAAGA83730.19777872734243074No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT76310.18025193698197647No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC70130.16565415201868705No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG64750.15294604795679434No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAGGCCTGCAGCCGGACGATCGC64600.15259173278778246No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAGGCCCCTAAGTGGTGACTCAG62810.14836357177090737No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC60690.14335591738220615No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT60070.14189141468362373No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG58070.13716721243013202No Hit
AGGTACCACCGCCGCTCAAGTGGCGTCCGGCTACAGCATCAGTAAAACCA58010.13702548636252723No Hit
TTCACCCGAGTTCTCTCAAGCGCCTTGGTATTCTCTACCTGACCACCTGT57010.1346633852357814No Hit
AGGCGAGGCTGAAAAGCGTAGTCGATGGGAAGCAGGTAAATATTCCTGCA48960.11564847116547723No Hit
CTCAGAGTAATAGAGGTGCCTGCAGGTTTAAGCAGGGGACTGGCATAATG45480.10742835924440165No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT44700.10558592036553986No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG43760.10336554530639877No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGCA43000.10157034845007193No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)