FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1700621
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT154190.9066687992209905No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC127410.7491969110107425No Hit
NNNNNNNN120150.7065066231688306No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT97040.5706150870770148No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG91590.5385679701708964No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC55720.3276450190842051No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC54860.32258804283846904No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG54840.32247043873973097No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA51900.3051826362252377No Hit
AGGCACTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTA50910.29936123333770426No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT45360.26672609593789565No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC42690.251025948756366No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG40170.23620783231537187No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC34070.20033858220026685No Hit
CGGCCCCCCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCA33930.1995153535091005No Hit
GGGCCACTTTTGGTAAGCAGAACTGGCGCTGCGGGATGAACCGAACGCCG32790.1928119198810317No Hit
TGGCTCCTCAGCCAAGCACATACACCAAATGTCTGAACCTGCGGTTCCTC32360.19028343175816362No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT29730.17481849277411016No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC28730.1689382878372077No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG28630.16835026734351746No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGACCTCAGATCAGACGTGGCG26810.15764829435835498No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA26510.15588423287728426No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG25110.1476519459656208No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT22790.13400987051200708No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT22630.13306903772210268No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG21510.12648320819277195No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG20960.12324909547747558No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT20260.11913295202164385No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG20250.11907414997227483No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG19690.11578123520760947No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG19030.11190029994925382No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCTAAACCTAGCCCCAAACCCAC18650.10966582207323089No Hit
CGGCCCCCCGGTTAAGCGACTAAGCGTACACGGTGGATGCCCTGGCAGTC18530.10896019748080259No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGGTTAAGCGACTAAGCGTACACG18460.10854858313521942No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG18150.10672571960477968No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG17670.10390322123506648No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC17470.10272718024768598No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGAGACACGGTCCAGACTCCTAC17180.10102192081598428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)