FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335433

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335433
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665877
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA732624.397803679383292No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT350542.1042369874846703No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA149860.899586223952909No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC142260.8539646084314747No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT141960.8521637551872077No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG105830.6352809961359692No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA95580.5737518436235088No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT90210.5415165705511271No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT84760.5088010699469409No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG78400.4706229811684776No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG63970.3840019401192285No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT63860.3833416272629972No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT58750.35266709366898036No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT56190.33729981265123415No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGGCGGCGGCTTCACGGTTTTT38250.22960878864405954No Hit
TGGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTT38050.22840821981454812No Hit
NNNNNNNN29180.17516299222571655No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC24980.14995104680597665No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT21340.1281006941088688No Hit
TAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGTA19830.11903639944605754No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)