FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1665877
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA486892.9227247870040824No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT264111.585411167811309No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC124730.7487347505247987No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT121310.7282050235401534No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG101560.6096488516259003No Hit
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA97270.5838966502328804No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT87880.527529943687319No Hit
AGGCCGAAGCCTTCGTTCGACTTGCATGTGTTAGGCATGCCGCCAGCGTT81860.4913928219190252No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG81330.48821131452082No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA70000.4201990903289978No Hit
CGGCCCCCCTTCGTGAAGCGAACCCGGCGAACTGAAACATCTCAGTAGCT51140.3069854497060707No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT49760.29870152478244194No Hit
AACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTG47900.28753623466798567No Hit
GAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGT41770.2507388000434606No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTGGGCGGCGGCTTCACGGTTTTT33090.19863411284266486No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30060.18044549507556681No Hit
TGGTGGTGCGGACTCTCAAGCGTGAGGTAAGGGCAATTCGTGGATGCCTT29230.1754631344330944No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC26030.15625403316091163No Hit
CAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTGT24430.14664948252482024No Hit
CAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAACT20800.12485915826918795No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)