FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2042426
Sequences flagged as poor quality0
Sequence length8-150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC158300.775058680216566No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG80630.3947756246737948No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC75110.3677489416997237No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC63060.3087504761494419No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT56950.27883507162560606No Hit
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT36570.1790517747032206No Hit
TGGCAATACCTTTTGGCTTCCAGGGAAGTTCAAGGCAATAGAGAAGGAGG28710.140568128294489No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26670.13058000632581057No Hit
TGGCTGGAGAGGAGCTGCCTGTGACCAGAGGGTTCTCATAGTCAGGAGAA26630.13038416079701295No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA20720.10144798391716517No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)