FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524485
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG218090.6187854395748599No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC204460.5801131229101556No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG167310.47470765232367285No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC165900.47070706784111727No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT143130.4061018843887831No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG139020.3944406062162274No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG128530.3646773925835973No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT126260.35823673529607875No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC114860.3258915841605228No Hit
NNNNNNNN108440.30767615694207806No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG73780.20933554831415085No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT70810.20090878525515074No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT63790.1809909816611505No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC61410.17423822203811337No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC58980.1673435977171133No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA58410.1657263401603355No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT51770.14688670827085376No Hit
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT46840.13289884905170543No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT46350.13150857501166838No Hit
AGAACGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAGCGAGGACTT44030.12492605302618681No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG42730.12123757087914971No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG41340.11729373227577929No Hit
TCTCCTCTCCTGCCCTCAAGTCCCCCAGTTTCCAATGACCCTCCACGGTT35350.10029834145981611No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG35290.10012810382226056No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)