FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335448

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335448
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3524485
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC175740.4986260404002287No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG155590.44145456712115383No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT154640.4387591378598576No Hit
CGGCCCCCCTTGAAAAGCCAGGGGATGACTTGTGGATAGGGGTGAAAGGC128640.3649894949191158No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG117150.33238898732722655No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG114320.3243594454225227No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC111250.3156489529675967No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT92050.2611729089498182No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTGAAAAGCCAGGGGATGACTTG87840.24922790138133658No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC61350.17406798440055782No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT60750.17236560802500223No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG57140.16212297683207616No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT56060.15905869935607614No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA49290.1398502192518907No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45440.12892663750874242No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT43960.12472744244903865No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT42280.11996078859748303No Hit
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT41970.11908122747011265No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC40480.1148536594708163No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)