FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335449

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335449
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3590480
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT89300.24871326396470667No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG82450.22963503486998954No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC80010.22283928611216328No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT78200.2177981774024643No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC71430.19894275974243No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC66460.1851005993627593No Hit
TGGCTGGAGAGGAGCTGCCTGTGACCAGAGGGTTCTCATAGTCAGGAGAA47420.13207147790824625No Hit
TGGCACCATGTTGTATTGTCTATTATGTCCAATAGGGGCATTTTCCAATG41540.1156948374590584No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG39870.11104364876005438No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG39100.10889908870123215No Hit
TGGCAATACCTTTTGGCTTCCAGGGAAGTTCAAGGCAATAGAGAAGGAGG38520.10728370579978165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)