FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335451

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335451
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3833890
Sequences flagged as poor quality0
Sequence length8-150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC755461.970479069561203No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG440431.1487810031065053No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC332910.8683347722548116No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT169420.4419010456742369No Hit
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT142280.3711113255727212No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT115210.30050418765274955No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT107690.2808896447211579No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC102030.2661265711848801No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT96190.2508940006103461No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC77420.201935892787743No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG70760.1845645023722642No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG68350.17827845869338974No Hit
NNNNNNNN65170.1699840110175305No Hit
TGGCAATACCTTTTGGCTTCCAGGGAAGTTCAAGGCAATAGAGAAGGAGG65060.16969709616081838No Hit
CGGCCCCCCGATACACGCGGCCAGCCAGCAGACAGAGGACTCTCATTAAG65020.16959276348565033No Hit
CGGCCCCCCAACACGGGAAACCTCACCCGGCCCGGACACGGACAGGATTG64250.16758435948866557No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCATGGCACATGCAGCGCAAGTAG61910.16148089799133516No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA61480.16035932173327874No Hit
CAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGGT60350.1574119236597816No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG57910.1510476304745311No Hit
TGGCGCAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCC48780.12723369736742576No Hit
TGGCGCCCCTGCTGGGCCACTGATTGTGCCTTATAACCTGCCTTTGCCTG44090.11500069120397298No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC42660.11127079806671553No Hit
TGGCTGGAGAGGAGCTGCCTGTGACCAGAGGGTTCTCATAGTCAGGAGAA41620.10855814851234646No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAACACGGGAAACCTCACCCGGCC40950.10681057620328178No Hit
TGGCACCATGTTGTATTGTCTATTATGTCCAATAGGGGCATTTTCCAATG39500.10302851672844031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)