FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4417149
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC744461.6853857544764734No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG422400.9562729262698633No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC293110.6635728158592794No Hit
CGGCACGTCCCACAGTTGTGTCCTGAGAAATTGCCGTTGCAGTGACATGT154170.3490260346662519No Hit
NNNNNNNN138050.31253190689288496No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT121960.27610569623075876No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT121710.2755397203037525No Hit
CGGCCCCCCTAGGGACCTGTATGAATGGCTCCACGAGGGTTCAGCTGTCT120740.273343733706968No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC99880.2261187023575614No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTATGGCACATGCAGCGCAAGTAG89200.2019402107558518No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAGGGACCTGTATGAATGGCTCC87320.19768407178476435No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT79010.17887103197107454No Hit
CGGCCCCCCGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGAC78440.17758060685750016No Hit
CGGCCCCCCGATACACGCGGCCAGCCAGCAGACAGAGGACTCTCATTAAG72590.16433677016555248No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGACCCACCAATCACATGCCTA63180.14303343627303494No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCCATGGCACATGCAGCGCAAGTAG61660.13959230263683656No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG59820.13542671981407012No Hit
GACTGTGAGTGCGTTCGTAGTTTGAGTTTGCTAGGCAGAATAGTAATGAG50250.11376116132826852No Hit
CAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGGT46990.10638083524010623No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC45940.10400373634667973No Hit
CGGCCCCCCACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATT45250.1024416427881423No Hit
TGGCAATACCTTTTGGCTTCCAGGGAAGTTCAAGGCAATAGAGAAGGAGG45050.10198886204653725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)