FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3745184
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT109330.291921571810624No Hit
AGGCGAGGCCGAAAGGCGTAGTCGATGGGAACAGGGTGAATATTCCCTGG104870.28001294462434956No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTACCTGGTTGATCCTGCCAGTAG102370.2733377051701599No Hit
CGGCCCCCCTACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAA74590.19916244435520392No Hit
NNNNNNNN59280.1582832779377462No Hit
TGGCCTCACTCAACTGTGCCTCCTGGGGCCAGATATGTGCTCCCACAGGG54110.14447888274648188No Hit
AGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT42660.11390628604629305No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAGTGCATGGATCCCCTGCCTGCC39960.10669702743576817No Hit
AGGCCCCCCACCACTGCCCCAGCTGCTGCAGCACCAGCCATGTGCTTCAT39500.10546878337619728No Hit
GGGCCGGCTAACCCTGCGCAGATTAACTTTACGCAGGAACCCTTGGACTT39320.10498816613549562No Hit
AGGCGATGAAAGACGTGACAGCCTGCGAAAAGCGTCGGGGAGGCGGCAAT38940.10397352973845879No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT38800.10359971632902415No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)