FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191424
Sequences flagged as poor quality0
Sequence length8-150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG164880.2663038422178807No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG144690.23369421961732875No Hit
CTTGAACTCCTGACCTCAAGTGATCTGCCCGGCCTGCATTTTTTATTTCT140500.2269267942237521No Hit
TAAAGACAGCCCTTCTCAAGATTCCTCCGCGCGCAGCGGATAGGGACCGA135720.2192064378081682No Hit
NNNNNNNN122150.19728902430200226No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACTGGTGGCACCCCACCCGCAA121500.1962391850404689No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG101390.16375877342595177No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC93340.1507569179561923No Hit
TGGCTCTTTTTTACAGGTTTTTGGTTGACACTTCTCTGCAGAATAGCACC92660.14965862457489584No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA80630.13022852254990128No Hit
CCTGGCCGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC80590.13016391705688385No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG79200.12791887617452788No Hit
CTACCTCAAATATACTCAAGACTTACAGTATCAATGGCAGTTCTATCGTT75380.12174905159136251No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGAACCGGGGGAACTGAAACATCT73470.11866413929977981No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC65620.10598531129510755No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCACACTGGTACTGAGACACGGACC65090.1051292885126265No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGGTCGCCTCCTAAAGGGTAAC63170.10202822484778946No Hit
GGAATTCCTGGTGTAGCGGTGAAATGCGCAGATATCAGGAGGAACACCGA62750.10134986717110636No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)