FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335470

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335470
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6191424
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG162330.262185242038019No Hit
TAAAGACAGCCCTTCTCAAGATTCCTCCGCGCGCAGCGGATAGGGACCGA130520.21080772371590123No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGACTGGTGGCACCCCACCCGCAA100240.16190136550170042No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG95460.15418100908611654No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCGCGGTCGCCTCCTAAAGGGTAAC93980.15179060584447132No Hit
CTTGAACTCCTGACCTCAAGTGATCTGCCCGGCCTGCATTTTTTATTTCT87440.14122760773612014No Hit
AGAATTCCAGGTGTAGCGGTGAAATGCGTAGAGATCTGGAGGAATACCGG83580.13499317765993735No Hit
CTACCTCAAATATACTCAAGACTTACAGTATCAATGGCAGTTCTATCGTT75240.12152293236580146No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC75190.12144217549952968No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA72490.11708130472085258No Hit
TGGCTCTTTTTTACAGGTTTTTGGTTGACACTTCTCTGCAGAATAGCACC63020.10178595424897408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)