FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3686666
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGCATAATGGGTCTGTGACTTAATGTATCAAGCAAGCTTAAGCCGTTA345960.9384088496218533No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG162770.44151002558951635No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA149160.4045932015539243No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT106490.288851770135944No Hit
GTGTCCCGCCCTACTCAAGTCCGACAATTTCACTTTCGCATACGGGGCTG103890.2817993276309815No Hit
CGCGCCTACTCCTGCTGCCAAAGCCTGGCCTCCCGGGTGTGGGAGAGAAG100750.27328214706729603No Hit
GTAATCCTGTCTGAACATAGGGGGACCACCCTCTAAGCCTAAATACTCGT97480.2644123443783624No Hit
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG87940.2385353053409232No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA87330.23688069383014354No Hit
GCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCATCGGCGTGGCCTTT77570.21040690965766903No Hit
GTACGCTGTCAGGCCTCAAGGACCCTCCAACTGCTTGTAGGCAATCCGTT69470.18843583877682438No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC68660.1862387316887399No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG67860.1840687493795207No Hit
AGAATGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAAGGCTT67290.18252263698420199No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTTCGTGAAGCGAACCCGGCGAAC64440.17479207500760852No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG63440.17207959712108445No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT57250.15528935900350072No Hit
GGGCCCCAACGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTAT54780.14858953862378635No Hit
GCGCCACTAAAGCCTCAAGGGCCCCAACGGCTAGTAGACATCGTTTACGG51550.13982823505031378No Hit
ACAGGCCATGTCTTGCAAGTCTATGTTTGGGTTCATTTTTCTTTGCATAA48890.13261304387215983No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT46920.1272694624357075No Hit
TTCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGT46280.1255334765883321No Hit
CGCTTTTCAGCGACTCAAGCGCCGCACGTCCATAGCGACCGCTCTGTTCG45770.12415011286620485No Hit
CGGCTAGTAGACATCGTTTACGGCATGGACTACCAGGGTATCTAATCCTG45650.12382461551982196No Hit
TCAGGACACTTCTCTCAAGCGCCTTGGTATACTCTACCTGACCACCTGTG45440.12325499516365193No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTCTGAACCAGTCACGTAGCGATA45200.12260400047088617No Hit
AGGCTGTTTACCAGACTGGATATCGCCAGTTTGGGAGGATAGTTAATTAA42070.11411394468606594No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT38590.10467452164096233No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC38230.10369802960181367No Hit
AAATCCAGTCATTGCTCAAGATCCAAAGCAACCCCCTGATCCACCTGTAG38080.10329115791883506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG37860.10269441278379979No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC37820.10258591366833882No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG37620.10204341809103401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)