FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335481

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335481
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1062474
Sequences flagged as poor quality0
Sequence length8-150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATG82350.7750777901388646No Hit
ACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCTT75660.7121115434354158No Hit
TGCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACG68830.6478276174287559No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAACTGAAGAGTTTGATCATGGC62780.5908850475399869No Hit
AACTAGGCAAAATGGTACCGTAACTTCGGGAGAAGGTACGCTGCCGATGG61550.5793082936617743No Hit
TGGCATAACCTTCTCCGTCCCTCCATCGCATCATAAACAAGTATCGGAAT48380.4553523192096936No Hit
TGTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGG44910.4226926964801021No Hit
NNNNNNNN40320.3794916393248211No Hit
TGGCGATGGTTGTCCAAGTGGAAGGATGTAGTTAGGTTTAGTAGGCAAAT33880.3188783913771066No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATAACGGTCCTAAGGTAGCGAAA31440.2959131235211403No Hit
CGGCCCCCCAAACTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG30950.29130124595990114No Hit
TACCAGGGTGGTATTTCAAGGATGGCTCCACAGACACTGGCGTGCCTGCT28150.2649476598956774No Hit
GTTGGGGTTCTAACTCAAGCTTAACAGAGCTGAGGACAATGTATGGTGGG24750.23294687681769152No Hit
GCGCCACTAAAGTCTCAAGGACCCCAACGGCTAGTAGACATCGTTTACGG20790.19567537652686087No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC20570.19360473762181477No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATTAAGTGGTAGGGGAGCATTGT17530.16499227275208617No Hit
TAACGCGATAAGTAGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC17010.1600980353401589No Hit
TGCGTCTTGTTGCAGATTTACCATCAAGTGTAAGTTAAGAGGTTTTTATG16310.15350963882410298No Hit
AGGCCACCTACGGTGAAAAGAAAGATGAATCCTAGGGCTCAGAGCACTGC14270.13430916897731146No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCTAAACTGAAGAGTTTGATCATGG14130.13299148967410027No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC13140.1236736146013926No Hit
CGGCGGTGGCAGTTCTGCTGTGGACACGCCTGCGCCCGGTCTATATTCCA12990.12226181534795205No Hit
TGGCATTGTATTAGCAAACTCATCACTAGACATCGTACTACACGACACGT11500.10823794276377587No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)