FastQCFastQC Report
Wed 8 May 2024
EGAF00007335493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4443987
Total Bases657.3 Mbp
Sequences flagged as poor quality0
Sequence length8-150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG658251.4812149540491455No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT539861.2148100343227826No Hit
NNNNNNNN489541.101578379954757No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC489011.1003857572040603No Hit
CGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAG254910.5736065384529703No Hit
TGGCCGGGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTC209500.47142352126592635No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG208040.4681381831224979No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG160960.36219727915495703No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG159500.35891194101152857No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT156290.3516886975591963No Hit
CGGCCCCCCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTG144840.32592354568093923No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA131770.29651301860243967No Hit
CGGCCCCCCACTGGCCGGGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAA127210.2862519624832386No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT122270.2751358183541041No Hit
CGGCCCCCCGGTGTGCACCGGTCTGCTCGTCCCTTCTACCGGCGATGCGC113280.25490623622436337No Hit
CGGCCCCCCAAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACG106330.23926712656900212No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATA105120.2365443463268457No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG103170.23215639469692417No Hit
CGGCCCCCCGGCGTAATGACTTCCCCGCTGTCTCCAACATAGACTCAGTG101530.22846601486457993No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA88080.19820039977614698No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT81440.18325886191836296No Hit
CGGCCCCCCTGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAG71520.16093656439589044No Hit
CTAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACT68190.15344329315094754No Hit
CGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGAC68050.15312826072623525No Hit
CGGCCCCCCGGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAG67180.1511705592298087No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG66680.15004544342726475No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT65010.14628755664676787No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA64390.14489241305161332No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT63350.14255217218232188No Hit
CGGCCCCCCACGCGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGA62930.14160707490818492No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG61320.13798420202399334No Hit
CGGCCCCCCTCAACCTGAGAGTTTGATCCTGGCTCAGAGCGAACGCTGGC59650.13422631524349643No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCATGGCACATGCAGCGCAAGTAGG56040.12610297914912894No Hit
CGGCCCCCCGAAGCAGCTTGCTGCTTCGCTGACGAGTGGCGGACGGGTGA55010.12378524059588833No Hit
CGGCCCCCCGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAACAG54810.12333519427487073No Hit
TGGCTCAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGG51410.1156844068175717No Hit
ACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGA49590.11158898529631163No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT47250.1063234433404058No Hit
TAACGCGTTAAGTCGACCGCCTGGGGAGTACGGCCGCAAGGTTAAAACTC45170.10164296160182287No Hit
TCAGAATTCATGCCCCCCCGGCCCCCCAAAAAAGCCGCAAAAAATAGTCG44860.1009453898042456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)