FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335494

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335494
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4443987
Sequences flagged as poor quality0
Sequence length8-150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT546681.2301566138694824No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG450571.0138868543044794No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC383010.8618612070647371No Hit
CGGCCATAGTCCCTCTAAGAAGCTGGCCGCGGAGGGTCACCTCCGCATAG219610.4941733627933655No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG167760.37749885406955513No Hit
CGGCCCCCCAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTG164590.3703656198814263No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT123130.2770710175344797No Hit
TGGCCGGGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAATTAGAGTGCTC110210.24799802519674335No Hit
CGGCCCCCCAAAAAAGCCGCAAAAAATAGTCGCAAACGACGAAAACTACG102000.22952362371897128No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG97520.21944258612817724No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT97070.21842998190588764No Hit
CGGCCCCCCGGGGCTGATTCTGGATTCGACGGGATTTGCGAAACCCAAGG78600.17686820415991317No Hit
CGGCCCCCCACTGGCCGGGTCGTGCCTCCGGTGCTGTTACTTTGAAGAAA78590.17684570184386228No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATA78520.17668818563150612No Hit
CGGCCCCCCAAATTGAAGAGTTTGATCATGGCTCAGATTGAACGCTGGCG74640.16795728700376486No Hit
CCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTTG70480.15859632352659897No Hit
AATGAATACCAAGTCTCAAGAGTGAACACGTAATTCATTACGAAGTTTAA68600.15436588810903362No Hit
GGGCACAACCTCCAAGTCGACATCGTTTACGGCGTGGACTACCAGGGTAT65260.14685011454803987No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT64630.14543246863683446No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT58480.13159354426554354No Hit
CGGCCCCCCGGTGTGCACCGGTCTGCTCGTCCCTTCTACCGGCGATGCGC55980.12596796525282364No Hit
CGGCCCCCCACGCGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGA53720.12088244182532487No Hit
CGGCCCCCCGGCGTAATGACTTCCCCGCTGTCTCCAACATAGACTCAGTG52840.11890223801284748No Hit
ATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTG51450.11577441608177522No Hit
TCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTA50700.11408674237795924No Hit
TGGCGCACGGGTGCGTAACGCGTGGGAATCTGCCCTCAGGTTCGGAATAA46680.10504081132550569No Hit
ACATCCTTGGCAAATGCTTTCGCAGTTGTTCGTCTTTCATAAATCCAAGA46400.10441074647608103No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT46310.10420822563162314No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG46110.10375817931060555No Hit
CGACCTTGATCTTCTGAGAAGGGTTCGAGTGAGAGCATACCTGTCGGGAC46060.10364566773035115No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)