FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335496

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335496
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2762034
Sequences flagged as poor quality0
Sequence length8-150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGATCTT717242.596781936790061No Hit
AGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATAG464861.6830350386707766No Hit
GTACGCGGTCAGGGCTCAAGGCCCCTCCCACTGCTTGTAGGCATCCGGTT396851.4368034571623665No Hit
TCACCTCTCCCGAACTCAAGATCTTCAGTATCAAAGGCAGTTCTGGAGTT250020.9052024703533701No Hit
CGGCCCCCCTTTGGTGAGGCGAACCCGGAGAACTGAAACATCTCAGTACC219560.7949214238492357No Hit
CTTGCATAATGGGTCTGTGACTTAGTGTATCAAGCAAGCTTAAGCCGTTA210400.76175745845272No Hit
CGGCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTC172770.6255172818292606No Hit
TTCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGT89850.3253037435455175No Hit
TCAACATAGTTCTCTCAAGCGCCTTGGTATACTCTACCAGTCCACCTGTG86100.31172679264628894No Hit
CGGCCCCCCGAACTGTAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCG77860.2818937058703839No Hit
CGGCCCCCCATAACGGTCCTAAGGTAGCGAAATTCCTTGTCGGGTAAGTT67690.24507301503167594No Hit
GGAACTGCGAGTGTAGAGGTGAAATTCGTAGATATTCGCAAGAACACCAG64840.23475453234826216No Hit
CAGAGCGAACGCTGGCGGCAGGCTTAACACATGCAAGTCGAGCGGGCATA54490.19728214786639123No Hit
AGATGTAACGGGGCTCAAGCCACGAGCCGAAGCTTAGGGTGTGATCCGCA53150.19243065074506688No Hit
CGGCCCCCCCTTTGGTGAGGCGAACCCGGAGAACTGAAACATCTCAGTAC52870.19141690507792447No Hit
CGGCCCCCCATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAG47720.17277122584298382No Hit
CGGCCCCCCACGCGCAGAACCTTACCAGCCCTTGACATCCCGGTCGCGGA47510.17201091659262704No Hit
GGGCCGACTCACTCTACGCCGATGAACGTTGCGTAGAAAACCTTGCGCTT45130.1633940784219166No Hit
CAGAACGAACGCTGGCGGCATGCCTAATACATGCAAGTCGAACGAGATCT45110.16332166801712072No Hit
AGAACGAACGCTGGCGGCATGCCTAACACATGCAAGTCGAACGAGATCCT44690.1618010495164071No Hit
AGGCAACCTTCTAGGTAACGACCACATCTACAACGTTATCGTCACAGCCC44100.15966494257492847No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT43470.15738401482385805No Hit
CTTGCGACCGTACTCCCCAGGCGGATAACTTAATGCGTTAGCTGCGCCAC38000.13757976911218328No Hit
CGGCCCCCCTATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT36050.1305197546445844No Hit
CGGCCCCCCCATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTT30590.11075171413530752No Hit
CGGCCCCCCAGCTAGCGTTGTTCGGAATTACTGGGCGTAAAGCGCACGTA30410.11010002049214457No Hit
CGGCCCCCCTCAAACTTGAGAGTTTGATCCTGGCTCAGAACGAACGCTGG28080.10166420833342385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)