FastQCFastQC Report
Sun 9 Jul 2023
EGAF00007335503

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00007335503
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3743396
Sequences flagged as poor quality0
Sequence length8-150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTTCTGGCCTGTGGCTTTCTGGGCAGGAACCTTCTGGGCTGGAGCCT192750.5149067851758137No Hit
NNNNNNNN181230.48413258976608403No Hit
CCCCCTCTACGAGACTCAAGCTTGCCAGTATCAGATGCAGTTCCCAGGTT148020.39541635456147306No Hit
CACTGATTTCATCCTCAAGTTTCCGCACAGTGCCCACCAGAAGTATGTCC138010.3686759295570119No Hit
CTTGGACTCCTGGGCTCAAGCGATCCTCCCACCTTGGCTTCCCAAAGTGC136880.3656572801808839No Hit
AGGCCCTCGCGCCTTTCCGGGTGAGGGCGGGCTCCGCAGCCCCCTCCTCC128980.34455344825928114No Hit
GCCCATCAACAGCCTCAAGAATTTTCAACAAATGCCCAGTCCTTTTCACT128960.3445000208366948No Hit
CTCAAACTCCAGACCTCAAGTGAGCTGTCTGCCTCGGCCTCCCAAAGTGC104510.279184996724899No Hit
ATGGGCTCATTATACTATGCTCTGTACTTGGGTATGTGTTTGGAATTCTT100890.26951463323677216No Hit
TCACTGATTTCATCCTCAAGTTTCCGCACAGTGCCCACCAGAAGTATGTC97400.2601915479954565No Hit
CGGCCCCCCTGTCACTTCTTTCCCTACCTCAGAGATTCTGTCAGGGGGTG80010.21373640405663732No Hit
CGGCCCCCCAGCAGACAGTCCTTGGGTGTCAGACATGTCATTTGGCTTCC77180.2061764237606708No Hit
CTTGACCTCCTGGGCTCAAGTCATCTTCTGGTCTTAGCCTCCCGAGTAGC76260.20371876232169933No Hit
CGGCCCCCCAGGTTTGACTCCCGTGCGGTGCGGCCCAGCAGCCACAAAGC73040.1951169472852992No Hit
CGGCCCCCCAAACACACACAAACCCTCCAACTACAATGTTAACATATGGA70410.18809124121519605No Hit
CGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCAATGTGTAGTCTTT67240.17962299473526178No Hit
AGGCGATGGAGTTACAGGAAGTGGTTCCCCTGACAGACACGCAGAACACC63520.16968549413420328No Hit
GCTCATTGCCTCTGATTCCCTTAGAAAGAGATGACTGGATGTCAATGACA61210.16351462682548146No Hit
CTTGAACTCCTGACCTCAAGCTGATCCTTCTGCCTTGGCCTCTCACAGTG61050.16308720744479077No Hit
CGGCCAAAGCCTTGGCAAGGCGCAGGGCCTACCGCCGGCTGAATCGGACG58130.1552868037471857No Hit
CGGCCATGCTTTACGGGCTTGTAGGTGATGGAGAACTCGCCCAGGTAGTG58040.1550463803455472No Hit
AAGAAAGAGCCTTGCTCAAGCTGTGTTTGGGATAACTCATAAAATTATGT57670.15405797302769997No Hit
CTTAAACTCCTGGGCTCAAGCGATTCTCCCGTCTCTGCCACCCAAACAGC57670.15405797302769997No Hit
AGGCGACGATCCCTAGCTGGTCTGAGAGGATGACCAGCCACACTGGAACT56810.15176059385648755No Hit
GCACACCATGGTCAGCCCAGAGCCTCCGGATGACGCCGGACAGGTCATCA56440.15077218653864033No Hit
CTGCCCAGGCCCCCTCAAGTCCAGTTTGCTGAGTAGCTGGCCATCCTCTA55040.14703226695759677No Hit
GTAATACATATAGTCTCAAGAATGGGAGTCCTCACATTTGACACGCTCTG54750.14625756933009493No Hit
TGGATCTAAATTGCCTAGCACATGGGCCACAGATGAGCTCAGTGAGCAGG52790.141021681916634No Hit
GCTGCCCAGGCCCCCTCAAGTCCAGTTTGCTGAGTAGCTGGCCATCCTCT52720.1408346859375818No Hit
TCACCACCAAAGAGCTCAAGATTCCTTGAAACCAACAAGGAGGATTTTTT52630.1405942625359433No Hit
CGGCCCCCCACAGGCATGAGCCACCGTGCCTGGCCATCTGGCAAGCTTTT52580.14046069397947747No Hit
CGGCCCCCCGGGGGCTCCATCCGATGGTTCTAATTTCATTCCCAATTTCC52030.13899143985835322No Hit
TGGCGTCCAGTGAGGATGATCAGAATGGTCCCGGGGGTAATGCTGGCTCG51800.13837702449861036No Hit
CGGCCCCCCGAACTTGCTAACCACAAAACCCGCCAGGCCGGTGCGGGAGC50650.13530494769989604No Hit
ACAAAATTCACAGCTCAAGTGGCAGCACTCTTTTAGTATACTAATGACAA50260.13426311295946247No Hit
CAAGTGCCGTACAATTCATCTAACTTCCGGAAAGCACTTTCAGTCCAAAT50070.1337555524448923No Hit
TACCACCAACTCGTCCAACTGACAAGCCCTTGCGCCTGCCTCTCCAGGAT49740.13287399997221774No Hit
CGGCTTCCGCTGGCCATGCTCCTGGTCTCTCCTTCCTGAGGTCACAGGCA49120.13121774987204132No Hit
CCTGGCAGATGCTCTCAAGAGTATCAACAATGCCGAAAAGAGAGGCAAAC48510.12958821348315808No Hit
GGGCCTCTGCTTGGTGGCCTCTCTGTTGCTCCCTGGGAGCAGGCCACAGC47980.12817238678462017No Hit
CGGCCCCCCGCTCTTCTGAGGATCCGGCAAGATGGCAGAAGTAGAGCAGA44150.11794103535933682No Hit
GGGCAGCCGTCCGGAGTTCAGCCAGCGCGGTGCCCAGGTTCTTGGCACAC43220.11545666020907219No Hit
GCACACCTCCCTGCCTCAAGGCCAACTAAGCCTGCATGCATTTTCTACGA43050.11500252711708833No Hit
CGGCCCCCCTAGCATATGCACATCGTGAAGGAAGAACAGACATGGCTTCG42880.11454839402510449No Hit
CGGCCCCCCAGCTGAACGATCCTGTTCAGAGGTTCTCCAGCACCCTGACG42400.1132661358830324No Hit
AGGCAGCTACAGAGGATGGGCAACTGTTGCGAGGGGTAGGAGCAGCAGCC41470.11078176073276778No Hit
GGGCATTCTGGACTCAGCTTGGGCTGCTGCTCCCGACCCCTACCCCCAGC41190.11003377681655908No Hit
CTCCAACTCCTGGGCTCAAGCAATCTGCTCGCCTTGGCCTCCTAAACTGC40730.10880494609707336No Hit
GCTAGCTGAGGACGAGGAGATGAACCGCATGCATGAGAGCATGAAGCTAT40670.10864466382931434No Hit
TATTTTTAGGATTCTGCATAGATTTCTTTTAAACTGGCATTCTTGCCTAA40510.10821724444862366No Hit
CGGCCCCCCGAGGAGGGCCTTCCAAGCTGGAGAAGTGGCAAGCGCAAAGT38410.10260736507705837No Hit
GGGCTGGCACAGGCCTCAGAACAGGTGCTGTTTGTGTGAATTCTCCTCCC38280.10226008683024718No Hit
AGGCTCTCCCGCCTTTGGCTGCATGGCTCCCTGTGCGTTTCTCGGGGGTC37600.10044355446231176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)